The invasive proteome of glioblastoma revealed by laser-capture microdissection.

  • NORLUX Neuro-Oncology Laboratory
September 28, 2019 By:
  • Daubon T
  • Guyon J
  • Raymond AA
  • Dartigues B
  • Rudewicz J
  • Ezzoukhry Z
  • Dupuy JW
  • Herbert JMJ
  • Saltel F
  • Bjerkvig R
  • Nikolski M
  • Bikfalvi A.

Background: Glioblastomas are heterogeneous tumors composed of a necrotic and tumor core and an invasive periphery. Methods: Here, we performed a proteomics analysis of laser-capture micro-dissected glioblastoma core and invasive areas of patient-derived xenografts. Results: Bioinformatics analysis identified enriched proteins in central and invasive tumor areas. Novel markers of invasion were identified, the genes proteolipid protein 1 (PLP1) and Dynamin-1 (DNM1), which were subsequently validated in tumors and by functional assays. Conclusions: In summary, our results identify new networks and molecules that may play an important role in glioblastoma development and may constitute potential novel therapeutic targets.

2019 Sep. Neurooncol Adv.1(1):vdz029.
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