Lecture series Translational Bioinformatics and Systems Biomedicine "MODELING THE EFFECT OF POST-TRANSLATIONAL MODIFICATIONS ON INTERACTIONS IN LARGE EUKARYOTIC PROTEIN COMPLEXES" - Prof Vera Van Noort

07/02/2019 15:00 to 07/02/2019 18:00 (Europe/Luxembourg)

Esch-sur-Alzette, Luxembourg

Speaker

Prof Vera Van Noort is Professor Computational Systems Biology, Centre of Microbial and Plant Genetics, Leuven, Belgium.


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Abstract

Protein post-translational modifications (PTMs) have an indispensable role in living cells as they expand chemical diversity of the proteome, providing a fine regulatory layer that can govern protein-protein interactions in changing environmental conditions. We have investigated the effects of acetylation and phosphorylation on the stability of subunit interactions in purified Saccharomyces cerevisiae complexes, namely exosome, RNA polymerase II and proteasome. I will present a computational framework that consists of conformational sampling of the complexes by molecular dynamics simulations, followed by Gibbs energy calculation by MM/GBSA. After benchmarking against published tools such as FoldX and Mechismo, we could apply the framework for the first time on large protein assemblies with the aim of predicting the effects of PTMs located on interfaces of subunits on binding stability. We discovered that acetylation predominantly contributes to subunits’ interactions in a locally stabilizing manner, while phosphorylation shows the opposite effect. Even though the local binding contributions of PTMs may be predictable to an extent, the long range effects and overall impact on subunits’ binding were only captured because of our dynamical approach. Employing the developed, widely applicable workflow on other large systems will shed more light on the roles of PTMs in protein complex formation.

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schedule

15.00 - 16.00: LECTURE

University of Luxembourg

Maison des Sciences Humaines

11, Porte des Sciences 

L-4366 Esch/Alzette

Blackbox room

17.00 - 18.00: 'meet THE SPEAKER'

University of Luxembourg

Biotech 2 Building

6, avenue du Swing

L-4367 Belvaux

Riken Conference Room (ground floor)

Registration required for the 'Meet the Speaker' - send us an email

programme 2019

About the Lecture & Workshop series - TRANSLATIONAL BIOINFORMATICS AND SYSTEMS BIOMEDICINE

The LIH lecture and workshops series in Translational Bioinformatics and Systems Biomedicine, supported by the FNR and jointly organized with the University of Luxembourg, are gathering internationally recognised speakers to address topics around Translational Bioinformatics and Systems Biomedicine.  Please find here  the current programme for 2019.

This lecture will be followed by a workshop especially dedicated to early-stage researchers.

Attendance to the lecture and workshop are free of charge. Should you be interested in registering for the workshop, please feel free to do so by sending us an email.

Where

Maison des Sciences Humaines
    11, Porte des Sciences
    Esch-sur-Alzette L-4362
    Luxembourg

When

From 07/02/2019 15:00
To 07/02/2019 18:00

Contact

Luxembourg Institute of Health
+352 26 970
+352 26 970 719
odoo@lih.lu